An team from SAFC's Cell Sciences and Development department, led by Dr. Nan Lin, recently published a paper in Biotechnology Progress profiling microRNAs in parental CHO DG44 and CHO K1 cell lines as well as 4 recombinant DG-44 derived CHO lines producing a recombinant IgG.
MicroRNAs play an important role in global gene regulation and it has been theorized that miRNAs could be useful biomarkers and cell engineering targets for recombinant protein production. More work needs to be done with miRNAs in CHO cells though.
This paper describes miRNAs that were found to be up- or down- regulated in IgG expressing CHO clones as compared to the parental, as well as differences in miRNA expression between the two parental CHO cell types. It is a first step toward potentially using miRNAs in cell engineering.
Posted by Bruce Lehr December 21st 2010.