An article in Biotechnology and Bioengineering details the knock out of the Bax/Bak genes in CHO using zinc finger nucleases (ZFNs) to yield cells that are apoptosis resistant. The cells continue to grow normally in a scaled down bioreactor model and were resistant to apotosis induction by starvation, staurosporine and sodium butyrate. Under the conditions of this investigation, the Bax/Bak deleted cells produced 2 to 5 fold more protein than wt CHO cells.
Posted by Bruce Lehr June 27th 2010.
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