If you had the zinc finger nuclease (ZFN) reagents in your hands right now to allow you to knock out – dhfr, GS, fut8, neu3, bax/bak, lactate dehydrogenase A (LDHA), or RACK 1? What would you do? You can directly affect selection, fucosylation, sialylation, apoptosis, waste product production – either alone or in combination (so-called trait stacking).
If you could do it, what genes would you want to manipulate? And what combinations would be interesting for your system and proteins of interest?
I’d like to get some feedback. It would be great if this could be discussed on the blog, but would even like private feedback if musts be (as my UK colleagues say).
These are the type of questions that we are pondering. And, we’re looking for market confirmation as well as potentially collaborators who might want to look at these – or even other genes of interest
Let’s hear from you out there.
Posted by Bruce Lehr February 4, 2010