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Bruce Lehr

We frequently use RNAi to validate new targets prior to shifting to ZFNs. RNAi can knock down a gene's expression but won't totally eliminate it and is not permanent. Zinc finger nucleases are very specific enzymatic scissors that can be designed to very specifically remove a single gene (they could also allow you to make a modification in a gene or to insert a gene)and the change is permanent. So as we get into designing platform cell lines for use in production, we go to ZFNs to create a stably modified set of traits. You can read more at this link to Sigma-aldrich site, http://bit.ly/axlyYd

Pharma Conduct Guy

Hi Bruce,

I'm not familiar with the ZFN paradigm. How does it compare to RNAi technologies?

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