With a license to zinc finger nuclease (ZFN) technology, we now can create cell lines with enhanced properties rapidly and efficiently - markedly so as compared to homologous recombination techniques. Given the power, what genes would you knock out?
From a recombinant protein producers viewpoint, using CHO cells, we surmise genes like dhfr, GS, or fut8 and combinations thereof could expected to be popular choices. How about an endogenous host cell protein that co-purifies with your therapeutic? Or elimination of an endogenous viral sequence from your host cell?
What would you knock out?
Posted by Bruce Lehr Jan 21, 2010